体外模拟胃肠消化对缢蛏抗氧化活性的影响

目的 基于体外模拟消化系统探究胃肠模拟消化对缢蛏的抗氧化活性的影响以及氨基酸含量的变化。方法 以新鲜缢蛏为原料,向体外模拟消化系统加入胃蛋白酶和胰蛋白酶,模拟在胃液和肠液下的胃消化、十二指肠消化和小肠消化过程,研究缢蛏在消化过程中的羟基自由基(·OH)清除率、二苯代苦味酰基自由基(2,2-diphenyl-1-picrylhydrazyl radical, DPPH·)清除率、还原力、水解度、游离氨基态氮及游离氨基酸含量的变化。结果 整个胃肠消化阶段,加酶组的·OH清除率、DPPH·清除率、还原力均高于对照组。肠消化产物的·OH清除率、DPPH·清除率、还原能力及水解度均高于胃消化产物,并且肠消化产物中的总游离氨基酸含量及具有抗氧化特征的氨基酸含量均显著高于胃消化产物(P<0.05)。在十二指肠消化阶段,·OH清除率、DPPH·清除率、还原力值达到最高。结论 体外模拟消化处理能够提高缢蛏的抗氧化活性,其中十二指肠消化阶段产物抗氧化活性最高。

Effects of simulated gastrointestinal digestion in vitro on the antioxidant activity of Sinonovacula constricta

Bioactive peptides have been the focus of research in China in recent years. Mariculture shellfish is one of the raw materials for the research of bioactive peptides because of its rich protein content. In order to explore the changes in protein activity during digestion and establish an effective in vitro simulation digestive system, this paper used fresh Sinonovacula constricta as the experimental material, washed it after shelled, and stirred it into miny meat. The gastric digestion, duodenal digestion and small intestinal digestion were simulated respectively in gastric juice and intestinal juice. The changes of ·OH clearance rate, DPPH· clearance rate, reducing ability, degree of hydrolysis and amino acid content of Sinonovacula constricta during digestion were studied. The results showed that both pepsin and trypsin could promote the antioxidant capacity of Sinonovacula constricta. During 0-10 hours of digestion, the scavenging rate of ·OH, DPPH·, reducing power, degree of hydrolysis and content of free amino acids after gastrointestinal digestion were all higher than before digestion, and the amino acids with antioxidant activity were significantly increased after gastric digestion. The results showed that the scavenging rate of ·OH, DPPH·, reducing power, degree of hydrolysis and content of free amino acids in intestinal digestion stage were higher than those in gastric digestion stage.