Enzymatic characterization of acid tolerance response (ATR) during the enhanced biohydrogen production process from Taihu cyanobacteria via anaerobic digestion |
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Authors: | Qun Yan Aijie WangChunfai Yu Nanqi RenYibo Zhang Guangsheng Zhang |
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Affiliation: | a School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China b State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China c Division of Science and Technology, Beijing Normal University-Hong Kong Baptist University United International College (UIC), Zhuhai 519085, China |
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Abstract: | Enhancement of biohydrogen production via anaerobic digestion from Taihu cyanobacteria (blue algae) after acid stress on anaerobic sludge, and the enzymatic characterization of the acid tolerance response (ATR) during the enhanced biohydrogen production process were investigated in this study. Comparing to those of the control, biohydrogen accumulation and hydrogen content increased by 1.9 and 1.7 times, when 12.5 and 7.5 g/L of acid stress on anaerobic sludge were performed respectively. Other than that, activities of hydrolytic enzymes, such as β-glucosidase, BAA-proteolytic enzyme and phosphatase were all improved during the enhanced biohydrogen process after appropriate acid stress. Significantly, activity of glutamate decarboxylase (GAD), the main microbial ATR stimulated by excessive acids, was increased consistently with the biohydrogen accumulation. Therefore, acid stress might be a practical approach to improving the biochemical traits of the anaerobic sludge. In turn, improved hydrolysis of organic substances would help the anaerobic sludge better survive excessive organic acids, and then enhance biohydrogen production from Taihu cyanobacteria. |
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Keywords: | Taihu cyanobacteria Biohydrogen Anaerobic digestion ATR Enzymatic characterization |
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