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藠头中抗菌活性成分的气相色谱-质谱分析及其机理的研究
引用本文:柏建山,吴玉杰,莫湘涛,郑胜华,陈威,夏立秋. 藠头中抗菌活性成分的气相色谱-质谱分析及其机理的研究[J]. 食品科学, 2004, 25(1): 146-149
作者姓名:柏建山  吴玉杰  莫湘涛  郑胜华  陈威  夏立秋
作者单位:湖南师范大学化学化工学院,湖南省湘阴华鑫实业股份公司,湖南师范大学生命科学学院
摘    要:本文采用有机溶剂萃取和Sephadex G-25凝胶柱纯化的方法,从藠头中提取抗菌物质。对藠头提取物进行测定,检验其抗菌活性,并用气相色谱-质谱(GC-MS)法对其化学成分进行分离鉴定,GC法测定各种化合物的相对百分含量,共鉴定出了9种成分,约占总提取物的82.12%。通过对藠头抗菌物质的分析,为其作为药用植物的开发利用提供了科学依据。同时,为其抗菌机理的研究奠定了基础。

关 键 词:藠头  气相色谱-质谱  抗菌物质  
文章编号:1002-6630(2004)01-0146-04

GC-MS Analysis of the Antibacterial Active Components fromAllium Chinense and Research of Its Mechanism
BAI Jian-shan,WU Yu-jie,MO Xiang-tao,ZHENG Sheng-hua,CHEN Wei,XIA Li-qiu. GC-MS Analysis of the Antibacterial Active Components fromAllium Chinense and Research of Its Mechanism[J]. Food Science, 2004, 25(1): 146-149
Authors:BAI Jian-shan  WU Yu-jie  MO Xiang-tao  ZHENG Sheng-hua  CHEN Wei  XIA Li-qiu
Affiliation:1.Deparment of Microbiology, College of Life Science ,Hunan Normal University;2.College of Chemistry and Chemical Engineering, Hunan Normal University;3.Xiangyin Huaxin Industrial Company of Hunan Province
Abstract:The antibacterial components from Allium chinense were extracted by organic solvment extraction and sephadexG-25 purification. The antibacterial activity of the extract was checked by sensitive bacteria. We isolate and identify the chemicalcomponents of the extract by GC-MS and determine the relative percentage content of the extract by GC, nine kinds ofcomponents were separated, identified and accounted for about 82.12%. It provided the scientific evidence for the plant to beutilized in medicine by analyzing the antibacterial components.At the same time it makes base for the research of the antibacterialmetabolism.
Keywords:Allium chinense  GC-MS  antibacterial components
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