紫杉醇分子印迹聚合物的制备及其吸附性能

采用分子印迹技术,以紫杉醇为模板分子,2-乙烯基吡啶(2-VP)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,在氯仿中制备了紫杉醇分子印迹聚合物.用紫外光谱法对紫杉醇和功能单体的相互作用进行了分析,通过平衡结合法和Scatchard模型评价了印迹聚合物的吸附特性.结果表明,紫...

Preparation of molecularly imprinted polymer and its adsorption characteristics for taxol

Molecularly imprinted polymer(MIP) was prepared through molecular imprinting technique by using taxol as the template molecule,2-vinylpyridine(2-VP) as the functional monomer,ethylene glycol dimethacrylate(EGDMA) as the cross-linker and 2,2′-azobisisobutyronitrile(AIBN) as the initiator in the porogen of chloroform.The interaction between taxol and 2-VP was analyzed by UV spectrometric detection.The polymer was investigated in equilibrium binding experiment and Scatchard analysis to evaluate the adsorption characteristics of MIP.The results indicated that the stable 1:2 template-monomer complex was formed prior to polymerization,and MIP exhibited specific molecule recognition capacity for taxol.Scatchard analysis revealed that the homogeneous binding sites were formed in the MIP,and the equilibrium dissociation constant(K D) and the apparent maximum number(Q max) of binding sites were estimated to be 29.89 mg/L and 5.66 mg/g,respectively.