| 酿酒酵母胞内代谢关键酶对乙醇的耐受性 |
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| 引用本文： | 聂好,董亮,侯德文,李明达,王晓丹,赵长新.酿酒酵母胞内代谢关键酶对乙醇的耐受性[J].大连工业大学学报,2011,30(5):318-321. |
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| 作者姓名： | 聂好 董亮 侯德文 李明达 王晓丹 赵长新 |
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| 作者单位： | 1. 大连工业大学生物工程学院,辽宁大连,116034
2. 华润雪花啤酒(盘锦)有限公司,辽宁盘锦,124010 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2007BAK36B01) |
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| 摘 要： | 分别添加2％、4％、6％、8％乙醇的酵母细胞糖酵解途径和三羧酸循环中葡萄糖-6-磷酸脱氢酶、丙酮酸激酶、异柠檬酸脱氢酶、苹果酸脱氢酶、乙醇脱氢酶、异柠檬酸裂解酶活力等关键代谢节点处的酶活力,以期考查关键酶对酒精的耐受性.实验结果表明,葡萄糖-6-磷酸脱氢酶对酒精的耐受能力较强,添加8％乙醇时的酶活力较无乙醇时提高23....
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| 关 键 词： | 酿酒酵母 酶活力 乙醇 耐受性 |
Tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae |
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| NIE Yu,DONG Liang,HOU De-wen,LI Ming-da,WANG Xiao-dan,ZHAO Chang-xin.Tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae[J].Journal of Dalian Dalian Polytechnic University,2011,30(5):318-321. |
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| Authors: | NIE Yu DONG Liang HOU De-wen LI Ming-da WANG Xiao-dan ZHAO Chang-xin |
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| Affiliation: | 1.School of Bioengineering,Dalian Polytechnic University,Dalian 116034,China; 2.China Resources Snow Breweries(Panjin) Company Limited,Panjin 124010,China |
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| Abstract: | The activity of Glucose-6-phosphate dehydrogenase,pyruvate kinase,isocitrate dehydrogenase,malate dehydrogenase,ethanol dehydrogenase and isocitrate lyase in the EMP pathway and TCA cycle were detected in the medium with 2%,4%,6%,8% ethanol to analyze the tolerance of key metabolic enzymes on ethanol in the vivo cell of Saccharomyces cerevisiae.The results show that glucose-6-phosphate dehydrogenase activity was increased by 23.65% when adding 8% ethanol;the activity of pyruvate kinase and alcohol dehydrogenase was decreased by 77.7% and 62.72% when adding 2% ethanol,and the activity of isocitrate dehydrogenase is only 0.003 U;malate dehydrogenase and isocitrate lyase activities were increased by 256.25% and 276.16% when adding 8% concentration of ethanol. |
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| Keywords: | Saccharomyces cerevisiae enzyme activity alcohol tolerance |
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