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硅酸盐细菌B925对烟草黑胫病菌的抑制作用及其16S rDNA序列分析
引用本文:赵秀香,陈华民,吴元华. 硅酸盐细菌B925对烟草黑胫病菌的抑制作用及其16S rDNA序列分析[J]. 烟草科技, 2007, 0(3): 56-60
作者姓名:赵秀香  陈华民  吴元华
作者单位:1. 沈阳农业大学植保学院,110161
2. 上海交通大学农业与生物学院,201101
基金项目:沈阳农业大学校科研和教改项目
摘    要:用从烟草根际土壤中分离的一株硅酸盐细菌B925及其发酵原液和发酵液的无菌滤液进行了对峙培养促进烟草种子萌发、离体叶片接种法和室内盆栽防治烟草黑胫病试验16S rDAN序列分析。结果表明:①B925发酵液处理能促进烟草种子萌发;②能减轻烟草黑胫病的扩展。对烟草黑胫病的预防作用为58.3%;③该菌为胶质芽孢杆菌。

关 键 词:硅酸盐细菌  烟草黑胫病
文章编号:1002-0861(2007)03-0056-05
修稿时间:2006-09-20

Inhibition of Silicate Bacteria B925 on Phytophthora parasitica var. nicotianae and its 16S rDNA Sequence Analysis
ZHAO XIU-XIANG,CHEN HUA-MIN,WU YUAN-HUA. Inhibition of Silicate Bacteria B925 on Phytophthora parasitica var. nicotianae and its 16S rDNA Sequence Analysis[J]. Tobacco Science & Technology, 2007, 0(3): 56-60
Authors:ZHAO XIU-XIANG  CHEN HUA-MIN  WU YUAN-HUA
Abstract:A strain of silicate bacteria,B925,isolated from the rhizosphere soil of tobacco plants in Fujian Province,it showed inhibition on Phytophthora parasitica var.nicotianae and other plant pathogens,and inhibited the former effectively either in the form of fermentation liquid or the aseptic filtrate of the liquid.The fermentation liquid at a dilution of 10 promoted the germination of tobacco seeds.The results in vitro inoculated test showed that the fermentation liquid of B925 prevented the spread of tobacco black shank disease,and the indoor pot experiment indicated the control effect was 58.3%.By 16S rDNA sequence analysis,strain B925 was identified as Bacillus mucilaginosus.
Keywords:16S rDNA
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