New finding of Phlebotom uses in the State of S?o Paulo with special reference to Lutzomyia longipalpis]

The removal by centrifugation of epididymal contents from mouse spermatozoa had no deleterious effect on fertilization in vitro, and, depending on the genotype of the gametes, was frequently associated with increased levels of fertility. Washing of the spermatozoa significantly improved the fertilization rate of F1 eggs with TO spermatozoa and of BALB/c eggs with BALB/c spermatozoa, but had no significant effect of F1 spermatozoa with F1 eggs.