GAMMA GLOBULIN ISOLATED FROM RABBIT ANTISERUM FOR RAPID DETECTION OF MEAT ADULTERATION |
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Authors: | MARY B. HELM M. O. WARNECKE R. L. SAFFLE |
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Affiliation: | Food Science Dept., University of Georgia, Athens, GA 30601 |
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Abstract: | SUMMARY –A simple, rapid method for the qualitative identification of animal proteins as to species was developed for fresh meat. Gamma globulin isolated from species-specific rabbit antiserum was mixed with a heated extract of skeletal muscle of beef, horse lamb and pork. The mixture was allowed to set for 30 min at room temperature and then centrifuged. The presence of a precipitin indicated a positive reaction. There were no cross reactions between meat samples from different species. This method requires a maximum time of only 1–2 hr, as compared with the 3 days required for double-gel diffusion. In addition, it is much more sensitive than gel diffusion. |
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