The effect of dietary lipid on the lipoprotein status of the mongolian gerbil |
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Authors: | Andrea B Leach Bruce J Holub |
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Affiliation: | (1) Department of Nutrition, College of Biological Science, University of Guelph, N1G 2W1 Guelph, Ontario, Canada |
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Abstract: | The Mongolian gerbil,Meriones unguiculatus, may be a suitable animal model for the investigation of dietary lipid effects on cholesterol metabolism. The effects of
dietary cholesterol, and its possible interaction with the type of dietary fat, on the lipoprotein status of this animal have
not been examined previously. In the present research, the effects of adding 0.5% cholesterol to diets high in saturated (19.5%
beef tallow: 0.5% safflower oil) or polyunsaturated (20% safflower oil) fats on the lipoprotein status of the gerbil were
determined after 11 and 22 days of feeding. Lipoproteins (VLDL, LDL and HDL) were separated by sequential ultracentrifugation.
Their cholesterol, phospholipid and protein concentrations were determined colorimetrically. In the absence of 0.5% cholesterol,
safflower oil lowered the concentration (mg/100 ml) of cholesterol in each of the VLDL, LDL and HDL relative to beef tallow
(BT) without greatly influencing the cholesterol distribution amongst them. The HDL carried the majority of the serum cholesterol
and the VLDL transported the smallest amount. However, inclusion of 0.5% dietary cholesterol resulted in a redistribution
of cholesterol amongst the lipoproteins so that the VLDL and LDL became the major and the HDL the minor carriers. Dietary
cholesterol also brought about a rise in the VLDL and LDL concentrations (mg/100 ml) of cholesterol, phospholipid and protein
and altered the VLDL and LDL compositions. No such changes were observed in the HDL, indicating that the HDL are relatively
resistant to any of the possible effects of cholesterol feeding measured in this experiment. The specific mechanisms responsible
for the changes observed in the lipoprotein status of the gerbil remain to be elucidated.
Presented in part at the Triennial Joint Meeting of the AIN/ASCN/CSNS, July 1982 |
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