| CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM |
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| 引用本文： | 要小未,李民乾,徐耀良,顾敏明,胡钧,张兰平,张家骅,陆长德,裘敏燕. CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM[J]. 核技术(英文版), 1992, 0(4) |
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| 作者姓名： | 要小未 李民乾 徐耀良 顾敏明 胡钧 张兰平 张家骅 陆长德 裘敏燕 |
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| 作者单位： | Institute of Nuclear Research Shanghai 201800 China，Institute of Nuclear Research Shanghai 201800 China，Institute of Nuclear Research Shanghai 201800 China，Institute of Nuclear Research Shanghai 201800 China，Institute of Nuclear Research Shanghai 201800 China，Institute of Nuclear Research Shanghai 201800 China，Institute of Nuclear Research Shanghai 201800 China，Institute of Biochemistry Research Shanghai 200031 China，Institute of Biochemistry Research Shanghai 200031 China |
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| 摘 要： | The large fragment of E.coli DNA polymerase I is imaged by scanning tunneling microscope. The specimen is deposited on highly oriented pyrolytic graphite surface, and then covered with pure paraffin oil in order to maintain hydration of the molecules. Images of the enzyme reveal an ellipsoid shape of 5.5-6.0nm wide and 7.0 -7.5 nm long. The conformation of the enzyme is in agreement with the model derived from X- ray crystallography studies.
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| 关 键 词： | STM E. coli DNA polymerase Ⅰ |
CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM |
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| Yao Xiaowei Li Minqian Xu YaoliangGu Minming Hu Jun Zhang LanpingZhang Jiahua. CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM[J]. , 1992, 0(4) |
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| Authors: | Yao Xiaowei Li Minqian Xu YaoliangGu Minming Hu Jun Zhang LanpingZhang Jiahua |
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| Abstract: | The large fragment of E.coli DNA polymerase I is imaged by scanning tunneling microscope. The specimen is deposited on highly oriented pyrolytic graphite surface, and then covered with pure paraffin oil in order to maintain hydration of the molecules. Images of the enzyme reveal an ellipsoid shape of 5.5-6.0nm wide and 7.0 -7.5 nm long. The conformation of the enzyme is in agreement with the model derived from X- ray crystallography studies. |
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