| HPLC和UV对比测定栀子和西红花中西红花苷的含量 |
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| 引用本文： | 陈阳,孙志勇,张浩,蔡乐,蒋李娟,许辉川.HPLC和UV对比测定栀子和西红花中西红花苷的含量[J].食品工程,2009(2):46-48. |
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| 作者姓名： | 陈阳 孙志勇 张浩 蔡乐 蒋李娟 许辉川 |
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| 作者单位： | 1. 遵义医学院珠海校区化学教研室,珠海,519041
2. 四川大学华西药学院生药学教研室,成都,610041 |
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| 摘 要： | 目的：对比考察HPLC和UV法测定西红花苷-1的线性关系及方法的精密度、稳定性、重现性和回收率。方法：采用HPLC和UV测定栀子和西红花药材西红花苷含量。结果：栀子药材紫外测定结果与高效液相色谱测定结果接近，而西红花药材高效液相色谱测定结果显著高于紫外结果。结论：两种方法都具有较好的线性关系，精密度、稳定性、重现性和回收率都符合要求（RSD〈3％）。
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| 关 键 词： | 栀子 西红花 西红花苷 高效液相 紫外 |
Comparative evaluation of HPLC and UV-visible methods to quantify crocins of gardenia and saffron |
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| CHEN Yang,SUN Zhi-yong,ZHANG Hao,CAI Le,JIANG Li-juan,XU Hui-chuan.Comparative evaluation of HPLC and UV-visible methods to quantify crocins of gardenia and saffron[J].Food Engineering,2009(2):46-48. |
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| Authors: | CHEN Yang SUN Zhi-yong ZHANG Hao CAI Le JIANG Li-juan XU Hui-chuan |
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| Affiliation: | 1Department of Chemistry;Zunyi Medical College;Zhuhai Campus;Zhuhai 519041;China;2West China School of Pharmacy;Sichuan University;Chengdu 610041;China |
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| Abstract: | Objective: comparatively evaluate the correlationships of crocin-1,precision,stability,repeatability and recovery of HPLC and UV-Visible analysis. Method:HPLC and UV-Visible analysis were employed to determine crocins of gardenia and saffron respectively. Results:Similar quantification results determined by two methods were obtained in the case of gardenia but significant higher value of saffron obtained by HPLC was observed in comparison with that of UV-Visible analysis. Conclusion: good correlationships,p... |
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| Keywords: | gardenia saffron crocin HPLC UV |
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