| 重组人白细胞介素-18的纯化、复性及其活性检测 |
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| 引用本文： | 陈文芳,张利宁,宋磊,刘志亭,刘应军.重组人白细胞介素-18的纯化、复性及其活性检测[J].粉末涂料与涂装,2005,18(3):238-240. |
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| 作者姓名： | 陈文芳 张利宁 宋磊 刘志亭 刘应军 |
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| 作者单位： | [1]山东大学医学院免疫学研究所，济南250012 [2]山东泉港药业有限公司，济南250011 |
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| 基金项目： | 山东省科学技术发展计划项目(021100103)(本项目属山东泉港药业有限公司). |
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| 摘 要： | 目的纯化和复性rhIL18,并测定其生物学活性。方法采用提取包涵体、分子筛层析及离子交换层析的方法纯化rhIL18,经稀释法复性,用IL18诱导PBMC产生IFNγ,ELISA法检测上清中IFNγ的水平。结果rhIL18纯度大于95%,复性回收率达30%以上,获得的产品可诱导PBMC产生大量的IFNγ,且呈剂量依赖关系。结论该纯化、复性方法为大量生产rhIL18打下了基础。
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| 关 键 词： | 白细胞介素-18 纯化 复性 |
| 修稿时间： | 2004年5月14日 |
Purification,Renaturation and Biological Activity of Recombinant Human Interleukin-18 |
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| CHEN Wen-fang,ZHANG Li-ning,SONG Lei,et al.Purification,Renaturation and Biological Activity of Recombinant Human Interleukin-18[J].Chinese Journal of Biologicals,2005,18(3):238-240. |
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| Authors: | CHEN Wen-fang ZHANG Li-ning SONG Lei |
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| Abstract: | Objective To purify and re-naturalize recombinant human interleukin-18(rhIL-18) and determine its biological activity.Methods Extract inclusion body of rhIL-18,purify by molecule sieve and ion-exchange chromatography and re-naturalize by dilution.The re-naturalized rhIL-18 was used for induction of secretion of IFN-γ by PBMC,and the IFN-γ level was determined by ELISA.Results The purity and recovery rate of rhIL-18 were more than 95% and more than 30% respectively.The re-naturalized rhIL-18 showed significant and dose-dependent activity in the induction of IFN-γ in PBMC.Conclusion The procedures for purification and re-naturalization laid a foundation of large-scale production of rhIL-18. |
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| Keywords: | Interleukin-18 Purification Renaturation |
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