一株嗜热毁丝霉菌株产纤维素酶条件优化

以一株嗜热的毁丝霉H127-1为出发菌株，对其产纤维素酶的液体发酵条件进行了研究。结果表明，最佳碳源为稻草粉，最佳氮源为黄豆粉。当添加一定量的金属离子时，Fe^2＋能显著促进H127-1产CMC酶以及β-葡萄糖苷酶，而Zn^2＋和CU^2＋则极大地抑制了其产酶；Ca^2＋、Ba^2＋和Mg^2＋都能促进H127-1产CMC酶，但在一定程度上抑制了其β-葡萄糖苷酶的分泌。发酵液初始pH值、装液量、接种量、发酵时间对H127-1的产酶也有一定影响，最适宜条件为初始pH4、装液量30～50mL／250mL、接种量6％～8％、最佳发酵时间为2～4d。

Optimization of Cellulase-producing Conditions of A Thermophilic Myceliophthora sp. H127-1 Strain

The cellulase-producing conditions of a thermophilic Myceliophthora sp.H127-1 strain were optimized as follows: the best carbon source was rice straw powder and the best nitrogen source was soybean meal,the addition of Fe2+ could remarkably improve CMCase production and β-glucosidase production but the addition of Zn2+ and Cu2+ would inhibit greatly CMCase production and β-glucosidase production,the addition of Ca2+,Ba2+ and Mg2+ in culture medium could improve CMCase production but would inhibit β-glucosidase production,the optimum initial pH value was 4,liquid medium volume was 30～50 mL/250 mL,the inoculating quantity was 6 %～8 %,and the optimum fermentation time was 2～4 d.