Dynamic confocal scanning laser microscopy methods for studying milk protein gelation and cheese melting |
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Authors: | M A E Auty M A Fenelon T P Guinee C Mullins D M Mulvihill |
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Abstract: | Dynamic confocal scanning laser microscopy (CSLM) methods were developed to enable observation of milk protein gelation and cheese melting. Protein aggregation and the formation of gel networks in renneted full-fat and low-fat milks and glucono-δ-lactone (GDL)-acidified skim milks were observed by CSLM and observations correlated with increases in shear modulus (G′) and dynamic viscosity (η*) as determined by dynamic amplitude oscillatory rheology. Confocal scanning laser microscopy observation of low-fat and full-fat cheeses showed changes in fat distribution and an increase in staining intensity during cheese melting. |
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Keywords: | confocal scanning laser microscopy dynamic studies protein gelation microstructure cheese melting |
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