超高效液相色谱-串联三重四极杆质谱法同时检测鸡肉中7种兽药残留

目的 建立超高效液相色谱-串联三重四极杆质谱(ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry,UPLC-MS/MS)同时检测鸡肉中7种兽药残留的分析方法.方法 采用固相萃取提取方法,运用Waters液相...

Simultaneous determination of 7 kinds of veterinary drug residues in chicken by ultra performance liquid chromatography tandem triple quadrupole mass spectrometry

Objective To establish a method for the simultaneous determination of 7 kinds of veterinary drug residues in chicken by ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry (UPLC-MS/MS). Methods Solid phase extraction and Waters liquid chromatography BEH C18 column was used for extraction and separation. Methanol (containing 0.2% formic acid)?0.2% formic acid aqueous solution was used as mobile phase for gradient elution, and tandem mass spectrometry was used for the simultaneous detection of 7 kinds of veterinary drug residues using electrospray ionization and multiple reaction monitoring (MRM) mode. External standard calibration was used for quantitative analysis. Results The linearity was good, correlation coefficients were all greater than 0.992, and the detection limit was 0.003?0.01 mg/kg. The average recoveries were 78.8%?94.1%, and the relative standard deviations (RSDs) were 1.58%?5.12%. Conclusion This method is simple, rapid, and accurate, which is suitable for quantitative analysis of 7 kinds of veterinary drug residues in chicken.