超高效液相色谱-串联质谱法测定凉茶中对乙酰氨基酚的不确定度评定

目的 采用超高效液相色谱-串联质谱法(UPLC-MS/MS)测定饮料中对乙酰氨基酚的含量,进行不确定度分析。方法 依据BJS 201713《饮料、茶叶及相关制品中对乙酰氨基酚等59种化合物的测定》,采用超高效液相色谱-串联质谱法(UPLC-MS/MS)测定对乙酰氨基酚的含量,并构建不确定度评定的模型,分析不确度分量的来源,对各分量进行不确定度评定。结果 饮料中对乙酰氨基酚含量为0.406mg/kg,其测量扩展不确定度为U=0.022mg/kg,k=2。结论 该研究可为实验室饮料中对乙酰氨基酚含量的测定结果质量提供参考,其中标准溶液配制、标准曲线拟合、重复性实验是标准不确定度的重要影响分量。

Uncertainty evaluation for determination of paracetamol in herbal tea by ultra performance liquid chromatography-tandem mass spectrometry

Objective To evaluate the uncertainty of determination of paracetamol in herbal tea by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods According to BJS 201713 Determination of 59 compounds including paracetamol in beverages, tea and related products, the content of paracetamol was determined by UPLC-MS/MS, the uncertainty evaluation model was established, the sources of uncertainty components were analyzed, and the uncertainty of each component was evaluated. Results Under the 95% confidence interval, when the content of paracetamol in herbal tea was 0.406 mg/kg, the expanded uncertainty was 0.013 mg/kg (k=2). Conclusion The preparation of standard solution, standard curve fitting and repeatability experiment are the important components that affect the standard uncertainty. This study can provide a reference for the quality control of the determination of acetaminophen content in herbal tea in laboratory.