| 共振散射光谱法研究槲皮素与DNA相互作用 |
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| 引用本文： | 黄国霞,阎柳娟,李军生,汪青.共振散射光谱法研究槲皮素与DNA相互作用[J].广西工学院学报,2010,21(3):19-22. |
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| 作者姓名： | 黄国霞 阎柳娟 李军生 汪青 |
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| 作者单位： | 广西工学院生物与化学工程系,广西,柳州,540006 |
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| 基金项目： | 广西工学院硕士科研基金项目 |
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| 摘 要： | 在BR缓冲液中,槲皮素存在微弱的共振散射现象,分别在288 nm、466 nm、562 nm处出现共振散射峰,随着DNA的加入,散射信号逐渐增强,且以上3处的峰值明显增加,并且562 nm处的增加值与DNA在3.024×10-7 mol/L～9.072×10-5 mol/L范围内呈现出良好的线性关系.在20℃时,通过Scatchard方程计算出槲皮素与DNA结合常数K=9.91×106 mol-1.L.
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| 关 键 词： | 槲皮素 鲱鱼精DNA 共振散射 |
Studies on the Interaction of Quercetin with Herring Sperm DNA by Resonance Scattering Spectra |
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| HUANG Guo-xia,YAN Liu-juan,LI Jun-sheng,WANG Qing.Studies on the Interaction of Quercetin with Herring Sperm DNA by Resonance Scattering Spectra[J].Journal of Guangxi University of Technology,2010,21(3):19-22. |
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| Authors: | HUANG Guo-xia YAN Liu-juan LI Jun-sheng WANG Qing |
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| Affiliation: | (Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou 545006,China) |
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| Abstract: | Quercetin has weak resonance scattering peaks at 288 nm,466 nm and 562 nm in B-R buffer solution.The intensity enhances obviously after adding DNA.The scattering intensity at 562 nm is linear to the DNA concentration in the range of 3.024×10-7 mol/L to 9.072×10-5 mol/L.The average binding constants are determined as 9.91×106 mol-1 L at 20 ℃. |
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| Keywords: | quercetin herring sperm DNA resonance scattering |
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