微生物絮凝剂的提纯及絮凝条件的优化

从污泥中分离并筛选出一株具有高絮凝活性的菌株,为了获得纯度高、性质稳定的絮凝剂,本文对其提纯工艺和絮凝条件进行了研究.实验结果表明,采用丙酮法对发酵液进行提纯,产量可达38.4 mg/L.该菌株产生高絮凝活性的最佳絮凝条件为.pH=7.0,絮凝剂投加量为1 mL,助凝剂为1％的CaCl2溶液、投入量为1.0 mL,先将溶液快搅1 min,再慢搅3 min后,对100 mL 1 g/L高岭土悬浮液的絮凝率可达90％以上,说明该微生物絮凝剂具有很好的应用前景.

Purification of microbial flocculant and optimization of flocculation conditions

In order to get the bioflocculant with high purity and stable character, this article research into the purification technology and optimization of flocculation conditions. A strain of high activity of flocculation bacteria was screened from the sludge. The results show that： purified the bioflocculant with acetone, the yield of which is 38.4 mg/L. The best conditions： 1.0 mL of microbial flocculant should be added when pH is 7.0. The best coagulant aid is 1.0% CaCl2 of 1.0 mL. The solution should be stired for 1 min at fast speed, and another 3 mins at low speed. The flocculation rate of kaolin suspension（100 mL 1 g/L） can up to 90.0%,which