| 发酵木糖生产乙醇菌株的筛选与鉴定 |
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| 引用本文： | 胡海军,梁运祥,葛向阳.发酵木糖生产乙醇菌株的筛选与鉴定[J].酿酒,2008,35(2):58-60. |
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| 作者姓名： | 胡海军 梁运祥 葛向阳 |
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| 作者单位： | 华中农业大学,农业部微生物学国家重点实验室,发酵工程研究室,武汉,430070 |
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| 摘 要： | 从花圃土和腐朽树皮中各筛选得到一株可以发酵木糖产生乙醇的菌株。乙醇产量分别可达到6.98g/L和6.05g/L。经过18SrDNA同源性的分析,菌株3鉴定为间型假丝酵母（Candida intermedia）,而菌株621则为热带假丝酵母（Candida tropicalis）。对其发酵木糖、葡萄糖和混合糖的产物蒸馏后用气相色谱分析,主产物为乙醇,纯度高,副产物少。两菌株为下一步的菌种基因改造工作提供了研究基础。
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| 关 键 词： | 木糖 乙醇 中型假丝酵母 热带假丝酵母 |
| 文章编号： | 1002-8110(2008)02-0058-03 |
| 修稿时间： | 2007年11月20 |
Strains Collection for Produce Ethanol by Xylose-Fermening |
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| HU Hai-jun,LIANG Yun-xiang,GE Xiang-yang.Strains Collection for Produce Ethanol by Xylose-Fermening[J].Liquor Making,2008,35(2):58-60. |
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| Authors: | HU Hai-jun LIANG Yun-xiang GE Xiang-yang |
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| Affiliation: | (Fermentation Engineering Research Laboratory., State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P.R. China) |
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| Abstract: | Two strains of yeast respectively isolated from garden soil and rotten cortex could effectively produce ethanol from xylose in 6.98 g/L and 6.05 g/L yield. Through 18S rDNA sequence analysis, one strain No.3 is characterized as Candida intermedia and the other strain No. 621 shows highly identical with Candida tropicalis. Results of Gas Chromatography analysis to the distilled products fermented from xylose, glueose or mixture of them, showed these two strains have high efficiency production of ethanol in all of them, which could be used as orginal strains for further genie alteration work. |
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| Keywords: | Xylose Ethanol： Candida intermedia Candida tropicalis |
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