| 紫外分光光度法测定壳聚糖包衣姜黄素脂质体的包封率 |
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| 引用本文： | 李嫄,胡江波,张敉,张景勋. 紫外分光光度法测定壳聚糖包衣姜黄素脂质体的包封率[J]. 激光杂志, 2014, 0(7): 123-124 |
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| 作者姓名： | 李嫄 胡江波 张敉 张景勋 |
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| 作者单位： | 重庆医科大学药物高校工程研究中心,重庆400016 |
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| 摘 要： | 目的:建立壳聚糖包衣姜黄素脂质体包封率的测定方法。方法:采用紫外分光光度法,在426 nm处测定吸光度值,计算壳聚糖包衣姜黄素脂质体中包封的药物量,由此计算出包封率。结果:姜黄素在426 nm处有最大吸收,浓度在0.5～8 mg/mL时与吸光度呈良好的线性关系,回归方程为A=0.1440C-0.008,R=0.9998(n=3);平均加样回收率为99.96%,RSD为0.29%。结论:本方法操作简单、快捷,能够应用于测定壳聚糖包衣姜黄素脂质体的包封率。
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| 关 键 词： | 姜黄素 脂质体 壳聚糖 包封率 |
Determination of the entrapment efficiency of chitosan coated curcumin liposomes by UV spectrophotometry |
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| Affiliation: | LI Yuan, HU Jiang-bo, ZHANG Mi, ZHANG Jing-qing (Medicine Engineering Research Center, Chongqing Medical University, Chongqing 400016, China) |
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| Abstract: | Objective：To establish the method determination of the entrapment efficiency of chitosan coated cur-cumin liposomes. Method：Entrapment efficiency was determined at the wavelength of 426 nm by UV-visible spec-trophotometry. Result：The curcumin had a good liner relationship range from 0.5-8 μg/mL. The regression equa-tion was as follows：A= 0.1440C- 0.008,R=0.9998（n=3）. The average recovery rate was 99.96%（n=9）,RSD =0.29%. Conclusion：The method was simple and rapid,which can be used for the determination of entrapment effi-ciency of chitosan coated curcumin liposomes. |
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| Keywords: | Curcumin Liposomes Chitosan Entrapment efficiency |
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