Quantitative competitive PCR for the detection of genetically modified soybean and maize |
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Authors: | Edgar Studer Claudio Rhyner Jürg Lüthy and P Hübner |
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Affiliation: | (1) University of Bern, Department of Chemistry and Biochemistry, Laboratory of Food Chemistry, Freiestrasse 3, CH-3012 Bern, Switzerland e-mail: philipp.huebner@ibc.unibe.ch, CH |
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Abstract: | The surveillance of food labelling concerning genetically modified organisms (GMOs) requires DNA-based analytical techniques.
Present assay systems allow the detection of GMO in food; however, they do not permit their quantitation. In this study, we
report the development of quantitative competitive polymerase chain reaction (QC-PCR) systems for the detection and quantitation
of the Roundup Ready soybean (RRS) and the Maximizer maize (MM) in food samples. Three DNA fragments that differ from the
GMO-specific sequences by an insertion were constructed and used as internal standards in the PCR. These standards were calibrated
by co-amplifying with mixtures containing RRS DNA and MM DNA, respectively. The calibrated QC-PCR systems were applied to
nine commercial food samples containing RRS DNA and to three certified RRS flour mixtures in order to elucidate whether these
food samples contain more or less than 1% RRS DNA. Finally, the GMO contents of four samples that were found to contain more
than 1% RRS were determined by QC-PCR using various amounts of standard DNA.
Received: 13 January 1998 / Revised version: 4 March 1998 |
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Keywords: | Quantitative competitive PCR Roundup Ready soybean Maximizer maize GMO Food analysis |
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