| 食管癌细胞中热休克蛋白70-多肽复合物的分离 |
| |
| 引用本文： | 陈兰英,高富荣,张玉焕,谢素嫩. 食管癌细胞中热休克蛋白70-多肽复合物的分离[J]. 中国生物制品学杂志, 2006, 19(5): 522-523 |
| |
| 作者姓名： | 陈兰英 高富荣 张玉焕 谢素嫩 |
| |
| 作者单位： | 河南平顶山工学院生物工程系 平顶山467001 |
| |
| 基金项目： | 河南省科技厅科技攻关项目 |
| |
| 摘 要： | 目的分离纯化癌细胞中热休克蛋白70(HSP70)-多肽复合物。方法以食管癌组织为材料,通过一系列的层析柱(ConA-Sepharose、ADP-Agarose、MonoQ及HSP70抗体亲和层析柱)进行分离及纯化。结果所分离纯化的HSP70-多肽复合物相对分子质量与预期相符。结论为提取肿瘤HSP70-多肽复合物提供了具体方法,并为研制多肽疫苗奠定了基础。
|
| 关 键 词： | 热休克蛋白 多肽复合物 食管癌 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005-12-19 |
Isolation of Heat Shock Protein 70 ( HSP70 ) -Polypeptide Compound from Oesophageal Carcinoma Cells |
| |
| CHEN Lan-ying, GAO Fu-rong, ZHANG Yu-huan, et al. Isolation of Heat Shock Protein 70 ( HSP70 ) -Polypeptide Compound from Oesophageal Carcinoma Cells[J]. Chinese Journal of Bilogicals, 2006, 19(5): 522-523 |
| |
| Authors: | CHEN Lan-ying GAO Fu-rong ZHANG Yu-huan et al |
| |
| Affiliation: | Department of Bioengineering, Pingdingshan College of Technology, Pingdingshan 467001, China |
| |
| Abstract: | Objective To isolate and purify HSP70-polypeptide compound from oesophageal carcinoma cells. Methods Isolate and purify HSP70-polypeptide compound from oesophageal carcinoma tissue by ConA-Sepharose, ADP-Agarose, MonoQ and HSP70 antibody affinity chromatography. Results The relative molecular weight of purified HSP70-polypeptide compound was consistent with that expected. Conclusion A method for the isolation of HSP70-polypeptide compound from tumor cells was developed. It provided a basis for the development of HSP70-polypeptide vaccine. |
| |
| Keywords: | Heat shock protein HSP70-polypeptide compound Oesophageal carcinoma |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
