| 不同品种茶叶单宁的含量与结构测定 |
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| 引用本文： | 李敏,王洁,杨志伟,林鹏,林益明.不同品种茶叶单宁的含量与结构测定[J].食品科学,2009,30(2):149-153. |
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| 作者姓名： | 李敏 王洁 杨志伟 林鹏 林益明 |
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| 作者单位： | 1.厦门大学生命科学学院 2.厦门大学湿地与生态工程研究中心 |
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| 摘 要： | 测定了本山、黄旦和铁观音三个品种茶树成熟叶片的总酚及可溶缩合单宁含量，并通过基质辅助激光解吸附飞行时间质谱(MALDI-TOF MS)研究了茶叶缩合单宁的基本结构。结果显示，三种茶叶均具有较高的总酚含量，大约为200mg/g；三种茶叶的缩合单宁是以(表)儿茶素(EC/C)和(表)棓儿茶素(EGC/GC)为基本结构单元的均聚物和杂聚物，且大部分聚合物的结构单元之间存在A 型和B 型两种连接方式，其中本山和黄旦缩合单宁的最高聚合度要大于铁观音。
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| 关 键 词： | 茶 多酚 缩合单宁 基质辅助激光解吸附飞行时间质谱 多聚物 |
| 收稿时间： | 2007-12-12 |
Contents and Structure Analysis of Tannins in Leaves of Three Cultivars of Camellia sinensis |
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| LI Min,WANG Jie,YANG Zhi-wei,LIN Peng,LIN Yi-ming.Contents and Structure Analysis of Tannins in Leaves of Three Cultivars of Camellia sinensis[J].Food Science,2009,30(2):149-153. |
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| Authors: | LI Min WANG Jie YANG Zhi-wei LIN Peng LIN Yi-ming |
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| Affiliation: | (1. School of Life Sciences, Xiamen University, Xiamen 361005, China；
2. Research Centre for Wetlands and Ecology Engineering, Xiamen University, Xiamen 361005, China) |
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| Abstract: | The contents of total polyphenols and extractable condensed tannins in leaves of three types of Camellia sinensis (Benshan, Huangdan and Tieguanyin) were determined. In addition, the structures of condensed tannins were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Three types of tea leaves have total phenolic contents as high as 200 mg/g. The majority of condensed tannins in the three types of C. sinensis are identified as the mixtures of procyanidins and prodelphinidins with a medium level of galloylation through the linkage of A-type and B-type interflavan bonds. Meanwhile the degree of polymerizations is higher in Benshan and Huangdan than in Tieguanyin. |
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| Keywords: | Camellia sinensis polyphenols condensed tannins MALDI-TOF MS polymers |
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