| 第一类整合酶表达载体的构建及鉴定 |
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| 引用本文： | 顿博影,曾冰冰,闫鹤,石磊. 第一类整合酶表达载体的构建及鉴定[J]. 现代食品科技, 2008, 24(1): 11-13 |
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| 作者姓名： | 顿博影 曾冰冰 闫鹤 石磊 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东,广州,510640;华南理工大学轻工与食品学院,广东,广州,510640;华南理工大学轻工与食品学院,广东,广州,510640;华南理工大学轻工与食品学院,广东,广州,510640 |
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| 摘 要： | Inti1基因是编码整合酶的基因,采用PCR方法对Inti1基因片断进行扩增,扩增子采用Ned1和BamH1双酶切,并克隆到pET19b载体上.把构建好的载体转化至E.coli BL21(DE3)中,在IPTG下进行诱导表达.经SDS-PAGE电泳鉴定以及使用特异性抗His-整合酶单克隆抗体的Western-Bloting证明该重组E.coli BL21(DE3)-Inti1在IPTG诱导下可表达整合酶,此为整合酶表达调控研究奠定了基础.
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| 关 键 词： | 整合酶 Inti1 不连续聚丙烯酰胺凝胶电泳 Western免疫印迹 食品 |
| 文章编号： | 1673-9078(2008)01-0011-03 |
| 收稿时间： | 2007-09-20 |
| 修稿时间： | 2007-09-20 |
Construction and Indentification of the Class1 Integrons Expression Vector |
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| DUN Bo-ying,ZENG Bing-bing,YAN He,SHI Lei. Construction and Indentification of the Class1 Integrons Expression Vector[J]. Modern Food Science & Technology, 2008, 24(1): 11-13 |
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| Authors: | DUN Bo-ying ZENG Bing-bing YAN He SHI Lei |
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| Affiliation: | (College of Light Industry and Food Science,South China University of Technology, Guangzhou 510640, China) |
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| Abstract: | Class1 integrase gene was amplified by PCR, then the amplicon was connected to pET19b vector after cutting by Ned1 and BamH1. The recombinant plasmids were induced to express integrase in E.coli BL21(DE3), and the integrase was identified by SDS-PAGE and Western Blot. The results showed that the integrase can be expressed in E.coli BL21(DE3). |
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| Keywords: | integrase Inti1 SDS-PAGE Western Blot food |
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