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食用仙人掌黄酮的超声提取条件及其抗氧化活性的研究
引用本文:彭爱红,叶鹏,陈素艳,郑姗姗,邓青莲. 食用仙人掌黄酮的超声提取条件及其抗氧化活性的研究[J]. 食品科学, 2006, 27(12): 527-530
作者姓名:彭爱红  叶鹏  陈素艳  郑姗姗  邓青莲
作者单位:集美大学生物工程学院; 厦门出入境检验检疫局;
摘    要:采用响应面分析法(RSA)研究了提取溶剂浓度、提取时间和料液比等食用仙人掌黄酮超声提取条件,比较了提取物粗品的体外抗氧化作用。结果表明,食用仙人掌黄酮超声提取的最佳工艺条件为:以仙人掌干粉为原料,以体积分数73.6%乙醇为溶剂,料液比1:10(m:V),提取时间18.8min。仙人掌黄酮粗提物表现出较强的清除羟自由基和1,1-二苯基苦基苯肼(DPPH)自由基的能力,其IC50值分别为4.62和7.92mg/L(以黄酮计),其清除羟自由基能力高于芦丁、硫脲、VC;清除DPPH自由基能力略低于VC而强于芦丁。

关 键 词:食用仙人掌   黄酮   超声提取条件   响应面   抗氧化活性  
文章编号:1002-6630(2006)12-0527-04
收稿时间:2006-08-17
修稿时间:2006-08-17

Study on Ultrasonic Extraction Conditions of Edible Cactus Flavones and Its Antioxidative Activity
PENG Ai-hong,YE Peng,CHEN Su-yan,ZHENG Shan-shan,DENG Qing-lian. Study on Ultrasonic Extraction Conditions of Edible Cactus Flavones and Its Antioxidative Activity[J]. Food Science, 2006, 27(12): 527-530
Authors:PENG Ai-hong  YE Peng  CHEN Su-yan  ZHENG Shan-shan  DENG Qing-lian
Affiliation:1.School of Bioengineering, Jimei University, Xiamen 361021, China; 2.Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361021, China
Abstract:The conditions of ultrasonic leaching flavones from edible cactus by response surface analysis and the antioxidative effect of cactus flavones crude extracts was compared in vitro. The results showed that the optimal ultrasonic leaching conditions were 73.8% ethanol, ratio of solid to liquid 1:10, extracting time 18.8min. cactus flavones extract had strong activity of scavenging hydroxyl free radical of Fenton reation and DPPH·, whose IC50 values were 4.62mg/L and 7.92mg/L respectively. Its scavenging hydroxyl free radical activity stronger than rutin, thiourea, VC, and its scavenging DPPH· activity weaker than VC while stronger than rutin.
Keywords:edible cactus  flavones   ultrasonic extraction conditions   response surface   antioxidative activity
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