香蕉及茶叶中纤维素酶产生菌的筛选

本实验从香蕉茎叶、茶叶及香蕉土壤、茶土壤环境中经初筛和复筛分离纯化出16株产纤维素酶的菌株，对其菌株的外在特性和产酶活力进行了研究和测定。结果从培养基来看，产酶菌株以从牛肉膏蛋白胨中性培养基里分离出来的为最多（5株）；体原料来源，以从土壤环境中分离出的菌株居多（10株）。菌株多为乳黄色（8株）和蓝绿色霉菌（3株）。水解圈以半透明的居多（12株），且16株菌株的水解圈与菌落直径比在1.0-2．0之间居多（10株）。产酶菌株的酶活和水解圈的透明状态基，树目符，即水解圈为透明状态的菌株酶活值较大，而水解圈为半透明状态的菌株酶活值相对较小；其中以查中香土10^-2-2-1的酶活值最大，为4．5。

Isolation of Cellulase-producing Bacteria from Leaves of Banana

In this study,16 powerful cellulase-producing bacteria were isolated from banana leave,tea leave and their soil. We investigated their characteristic and cellulase-producing activity. The result are as follow,the 16 powerful cellulase-producing bacteria are mostly isolated from the beef and peptone neutral culture,soil,most of them are yellow or blue-green mildew; hydrolyse zones are mostly translucence and the scale of D/d is mostly between 1.0 and 2.0.The cellulase-producing activity of these bacteria are accordance with the state of their hydrolyse zones. The cellulase-producing activity of Chazhong-xiangtu 100-2-1 is the most,reach 4.5.