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固定化微生物细胞降解邻苯二甲酸二丁酯
引用本文:孙晓然,单忠键. 固定化微生物细胞降解邻苯二甲酸二丁酯[J]. 中国化学工程学报, 2007, 15(2): 167-171. DOI: 10.1016/S1004-9541(07)60053-6
作者姓名:孙晓然  单忠键
作者单位:[1]School of Chenmical and Environmental Engineering,'ChinaUniversity of Mining and Technology, Beijing 100083,China [2]Department of Chemical Engineering, Hebei Polytechnic University, Tangshan 063009, China
基金项目:Supported by the National Natural Science Foundation of China (No.29637010, 50325824).
摘    要:The biodegradation of di-n-butyl phthalate (DBP) using immobilized microbial cells was carded out in an internal airlift loop reactor with ceramic honeycomb supports. A strain that is capable of degrading DBP was isolated from the activated sludge and identified as Bacillus sp. using 16S rDNA sequential analysis. Bacillus sp. could be rapidly attached onto the ceramic honeycomb supports. The immobilized cells could effectively degrade DBP in batch and continuous experiments. When the influent concentration of DBP was 50mg·L^-1, the effluent DBP reached less than lmg.L i with 6h hydraulic retention time (HRT) in continuous experiment. The immobilized microbial cells could grow and accumulate through the biodegradation of DBP, and the rate of degradation is accordingly increased. The possible pathway of DBP biodegradation using immobilized cells was tentatively proposed.

关 键 词:固定化微生物细胞  邻苯二甲酸二丁酯  生物反应器  污染物  去除  生物降解
收稿时间:2006-03-13
修稿时间:2006-03-132006-11-27

Removal of di-n-butyl phthalate using immobilized microbial cells
SUNXiaoran,SHANZhongjian. Removal of di-n-butyl phthalate using immobilized microbial cells[J]. Chinese Journal of Chemical Engineering, 2007, 15(2): 167-171. DOI: 10.1016/S1004-9541(07)60053-6
Authors:SUNXiaoran  SHANZhongjian
Affiliation:1.School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing 100083, China;2.Department of Chemical Engineering, Hebei Polytechnic University, Tangshan 063009, China
Abstract:The biodegradation of di-n-butyl phthalate (DBP) using immobilized microbial cells was carried out in an internal airlift loop reactor with ceramic honeycomb supports. A strain that is capable of degrading DBP was isolated from the activated sludge and identified as Bacillus sp. using 16S rDNA sequential analysis. Bacillus sp. could be rapidly attached onto the ceramic honeycomb supports. The immobilized cells could effectively degrade DBP in batch and continuous experiments. When the influent concentration of DBP was 50mg.L-1, the effluent DBP reached less than 1mg.L-1 with 6h hydraulic retention time (HRT) in continuous experiment. The immobilized microbial cells could grow and accumulate through the biodegradation of DBP, and the rate of degradation is accordingly increased. The possible pathway of DBP biodegradation using immobilized cells was tentatively proposed.
Keywords:biodegradation   phthalic acid ester   priority pollutant   bioreactor   immobilized cells
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