The dynamic changes of ascorbic acid,tocopherols and antioxidant activity during germination of soya bean (Glycine max) |
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Authors: | Linzhen Wang Hong Wang Qinrun Lai Tong Li Xiong Fu Xinbo Guo Rui Hai Liu |
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Affiliation: | 1. School of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China;2. Department of Food Science, Cornell University, Ithaca, NY, USA |
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Abstract: | The objective of this investigation was to study the dynamic changes of ascorbic acid, tocopherols and their antioxidant activity of soya bean sprouts during germination. Results showed that the expression levels of genes related to biosynthesis of ascorbic acid and tocopherols dramatically increased during germination. The expression levels of most genes were up to the peak at day 3 and kept constantly until the end of germination. The contents of ascorbic acid, α‐tocopherol, γ‐tocopherol, δ‐tocopherol and total tocopherols increased during soya bean sprouts germination and reached peak levels on day 5, of 74.42 ± 1.64 mg/100 g DW, 7.64 ± 0.13 mg/100 g DW, 15.84 ± 1.85 mg/100 g DW, 12.37 ± 1.06 mg/100 g DW, 35.85 ± 2.81 mg/100 g DW, respectively. The antioxidant activity of soya bean sprouts increased during germination and reached peak level on day 5 to 311.01 ± 49.01 μmol ASA equiv./100 g DW. Therefore, germination significantly increased the biosynthesis of ascorbic acid, tocopherols and antioxidant activity of soya bean sprouts. |
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Keywords: | Antioxidant activity ascorbic acid biosynthesis germination soya bean tocopherols |
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