Acid‐soluble and pepsin‐soluble collagens from grass carp (Ctenopharyngodon idella) skin: a comparative study on physicochemical properties |
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Authors: | Ye Chen Ran Ye Yan Wang |
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Affiliation: | Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, China |
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Abstract: | In this study, acid‐soluble (ASC) and pepsin‐soluble (PSC) collagens with triple helical structures were successfully extracted from the skin of grass carp (Ctenopharyngodon idella) by two different extraction approaches. SDS‐PAGE pattern revealed that ASC and PSC are type I collagens with typical α1, α2 and β‐chains. In addition, the intensity of χ‐chain (trimer) in ASC was higher than that of PSC, representing the presence of the high proportion of intra‐ and intermolecular cross‐links of extracted collagens with large molecular weight using the acid method. Differential scanning calorimetry (DSC) results demonstrate that Td (69.04 °C) of ASC was higher than Td (62.20 °C) of PSC. Both ASC and PSC had the highest solubility at acidic pHs or at a low concentration of NaCl (<2%, w/v). The results of FTIR suggested the ASC and PSC maintained in the helical secondary structure at high degree. |
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Keywords: | Acid‐soluble collagen fish skin grass carp pepsin‐soluble collagen |
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