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Study of pulsed light inactivation and growth dynamics during storage of Escherichia coli ATCC 35218, Listeria innocua ATCC 33090, Salmonella Enteritidis MA44 and Saccharomyces cerevisiae KE162 and native flora in apple,orange and strawberry juices
Authors:Mariana Ferrario  Stella Maris Alzamora  Sandra Guerrero
Affiliation:1. Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina;2. Scholar of Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Argentina;3. Member of Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Av. Rivadavia 1917 ‐ C.A.B.A, Argentina
Abstract:The response of some inoculated strains and native flora to PL treatment (Xenon lamp, 3 pulses s?1, 10 cm distance from the lamp, 71.6 J cm?2) in apple, orange and strawberry fresh juices with different absorbance, turbidity and particle size was investigated. Microbial growth dynamics during 12‐day storage (5 °C) of PL‐treated juices was also evaluated. PL treatments provoked 0.3–2.6 log reductions for inoculated microorganisms and 0.1–0.7 for native flora. High turbidity and particles with high UV absorbance seemed to play a major role in the PL efficiency compared to particle size. Cold storage of PL‐processed juices provoked an increase in Salmonella Enteritidis and Listeria innocua inactivation, achieving 5.0–8.0 log reductions, while no recovery of Escherichia coli and retardation for yeast growth was observed, compared to untreated samples. This study gives valuable information regarding the influence of juice variables on PL effectiveness and emphasises the beneficial effect of a postcold storage on microbial safety of PL‐treated juices.
Keywords:Food safety  fruit juices  physical preservation methods  pulsed light  shelf life
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