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Aeromonas biofilm on stainless steel: efficiency of commonly used disinfectants
Authors:Sara Craveiro  Cynthia Alves‐Barroco  Maria Teresa Barreto Crespo  António Salvador Barreto  Teresa Semedo‐Lemsaddek
Affiliation:1. Faculdade de Medicina Veterinária da Universidade de Lisboa, Centro de Investiga??o Interdisciplinar em Sanidade Animal, Avenida da Universidade Técnica, Lisboa, Portugal;2. ITQB ‐ Instituto de Tecnologia Quí?mica e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal;3. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
Abstract:Aeromonas spp. are ubiquitous bacteria widely distributed among aquatic environments that have the ability to form biofilms. This aptitude allows them to persist in water distribution systems, contaminating drinking water, food processing surfaces and ultimately food. For this study, the biofilm‐forming ability of aeromonads was evaluated after 48‐h incubation on stainless steel discs at both 4 and 20 °C. Subsequently, disinfectants based on amphoteric surfactants and chlorine compounds were evaluated regarding the capacity to eradicate preformed biofilm and inhibit biofilm formation. Results obtained demonstrated that all strains under analysis were able to form biofilm at both room and refrigeration temperatures. The chlorine‐based disinfectant demonstrated to be efficient in removing preformed biofilm, but both were unsuccessful in preventing biofilm formation, highlighting the importance of adequate cleaning and disinfection procedures, with emphasis on food processing surfaces.
Keywords:   Aeromonas     biofilm  disinfectants  food safety  stainless steel
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