| Abstract: | A specific to troponin T (as compared to f1 histone) proteinkinase is isolated using affinity chromatography on Sepharose 4B with covalently bound dephosphorylated troponin T. Homogeneous proteinkinase has a molecular weight of 175.000 and phosphorylates troponin T at a rate exceeding that of histone phosphorylation, while crude proteinkinase preparation has troponin T phosphorylation rate 15--20 times as low as that of histone phosphorylation. The enzyme isolated is capable of phosphorylating troponin T, but not troponin I, in the whole troponin complex. |
