在植物基因工程中应用的高压脉冲放电基因枪

本文介绍了一种用于植物基因工程领域的外源基因导入技术和高压放电基因枪的工作原理及关键结构，采用高速摄影法获得了射弹的运动速度。当储能为500J时，测得第一放电电流峰值为19．3kV，电流上升时间为9μs。生物学试验结果表明，高压放电基因枪能有效地将外源基因导入植物细胞并得到表达。

High voltage discharge gene gun used in plant gene engineering

This paper describes a technique of introducing foreign gene into pla nt cells and the principles of the high-voltage discharge gene gun and its key s tructures. The velocity of the macroprojectile are measured with high speed phot ography. The main specifications are: when storing energy is 500J，the first max discharge current is 19.3kA and the rise time is 9μs. The biological tests pro v e that the foreign gene can be introduced into plant cells and can be expressed effectively using the high voltage discharge gene gun.