Differentially expressed proteins during fat accumulation in bovine skeletal muscle |
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Authors: | Qiankun Zhang Hong-Gu Lee Jung-A Han Eun Bae Kim Sang Kee Kang Jinlong Yin Myunggi Baik Yannan Shen Sang-Hoon Kim Kang-Seok Seo Yun-Jaie Choi |
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Affiliation: | 1. Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea;2. Department of Animal Science, Pusan National University, Gyeongnam 627-706, Republic of Korea;3. Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea;4. Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea;5. Department of Animal Science and Technology, Sunchon, Sunchong University, 540-742, Republic of Korea |
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Abstract: | The objective of this study was to identify the proteins involved in bovine intramuscular fat (IMF) development. Global proteins were monitored in bovine skeletal muscle at muscle-developing versus IMF-increasing stages and with higher versus lower IMF scores, respectively. We identified two differentially expressed (two-fold or more) proteins at the IMF-increasing stage, up-regulated heat shock protein beta 1 (HSPB1) and down-regulated ATP synthase D chain (ATP5H), and two down-regulated proteins with higher IMF scores, carbonic anhydrase 2 (CA2) and myosin light chain 3 (MYL3). In vitro, after adipogenic differentiation, the mRNA expression of HSPB1 and ATP5H did not be changed, but that of CA2 and MYL3 decreased significantly (P < 0.05). After myogenic differentiation, the mRNA expression of HSPB1 increased significantly (P < 0.05), but expression of other genes did not vary. We suggested that CA2 and MYL3, which expressed down during adipogenic differentiation, could be indicative markers for negative regulation of IMF development. |
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Keywords: | Adipogenesis Bovine Bovine embryonic fibroblasts Intramuscular fat Myogenesis Proteomics |
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