Effect of cooking methods on fatty acids,conjugated isomers of linoleic acid and nutritional quality of beef intramuscular fat |
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Authors: | Cristina MM Alfaia Susana P Alves Anabela F Lopes Maria JE Fernandes Ana SH Costa Carlos MGA Fontes Matilde LF Castro Rui JB Bessa José AM Prates |
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Affiliation: | 1. CIISA, Faculdade de Medicina Veterinária, Avenida da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal;2. REQUIMTE, Unidade de Produção Animal, L-INIA, INRB, I.P., Fonte Boa, 2005-048 Vale de Santarém, Portugal;3. iMed.UL, Faculdade de Farmácia, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal |
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Abstract: | The effect of boiling, microwaving and grilling on the composition and nutritional quality of beef intramuscular fat from cattle fed with two diets was investigated. Longissimus lumborum muscle from 15 Alentejano young bulls fed on concentrate or pasture was analyzed. Cooking losses and, consequently, total lipids, increased directly with the cooking time and internal temperature reached by meat (microwaving > boiling > grilling). The major changes in fatty acid composition, which implicated 16 out of 34 fatty acids, resulted in higher percentages in cooked beef of SFA and MUFA and lower proportions of PUFA, relative to raw meat, while conjugated linoleic acid (CLA) isomers revealed a great stability to thermal processes. Heating decreased the PUFA/SFA ratio of meat but did not change its n−6/n−3 index. Thermal procedures induced only slight oxidative changes in meat immediately after treatment but hardly affected the true retention values of its individual fatty acids (72–168%), including CLA isomers (81–128%). |
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Keywords: | CLA conjugated linoleic acid FA fatty acids FAME fatty acid methyl esters MUFA monounsaturated fatty acids PUFA polyunsaturated fatty acids SFA saturated fatty acids TBARS 2-thiobarbituric acid reactive substances TFA trans fatty acids TR true retention |
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