Synthesis,characterization and cytotoxicity of novel modified poly[(maleic anhydride)‐ co‐(vinyl acetate)]/noradrenaline conjugate |
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| Authors: | Gulderen Karakus Zubeyde Akin Polat Ali Fazil Yenidunya Haci Bayram Zengin Can Bulent Karakus |
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| Affiliation: | 1. Research Center of the Cumhuriyet University School of Medicine, CUTFAM‐RCCUSM, 58140 Sivas, Turkey;2. Department of Parasitology, School of Medicine, Cumhuriyet University, 58140 Sivas, Turkey;3. Department of Molecular Biology and Genetics, Science Faculty, Cumhuriyet University, 58140 Sivas, Turkey;4. Department of Chemistry, Science Faculty, Cumhuriyet University, 58140 Sivas, Turkey;5. SIBESKI Management, Sivas, Turkey |
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| Abstract: | Poly(maleic anhydride)‐co‐(vinyl acetate)] (MAVA) copolymer was synthesized by free radical polymerization reaction, in methyl ethyl ketone at 80 °C, using benzoyl peroxide as the initiator. The copolymer was then modified with a biomolecule, noradrenaline (NA). The modification reaction was performed at 70 °C in dimethylformamide containing triethylamine as the catalyst. The modified polymer was named MAVA/NA. Structural characterization of the copolymer and the modified product was carried out by Fourier transform infrared (FTIR) and 1H NMR and 13C NMR spectroscopy. The FTIR, 1H NMR and 13C NMR spectra confirmed that NA was successfully covalently bound to the MAVA copolymer backbone. Surface morphology was visualized by atomic force microscopy. The cumulative release of NA from MAVA/NA was determined in phosphate buffered saline solution for 7 days at 37 °C and compared with MAVA. Cytotoxicity of the MAVA/NA was evaluated by using a mouse fibroblast cell line (L929). Results obtained indicated that MAVA/NA had almost no toxicity and no negative effect on cell viability at 250 µg mL?1 concentration. © 2012 Society of Chemical Industry |
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| Keywords: | atomic force microscopy copolymer modification drug release cytotoxicity noradrenaline poly[(maleic anhydride)‐co‐(vinyl acetate)] |
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