Fibroblasts Cultured on Nanowires Exhibit Low Motility,Impaired Cell Division,and DNA Damage |
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Authors: | Henrik Persson Carsten Købler Kristian Mølhave Lars Samuelson Jonas O Tegenfeldt Stina Oredsson Christelle N Prinz |
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Affiliation: | 1. Division of Solid State Physics, Lund University, Box 118, 22100 Lund, Sweden;2. The Nanometer Structure Consortium, Lund University, Box 118, 22100 Lund, Sweden;3. Center for Electron Nanoscopy, Technical University of Denmark, ?rsteds Plads 345E, 2800 Kongens Lyngby, Denmark;4. Department of Micro‐ and Nanotechnology, Technical University of Denmark, ?rsteds Plads 345E, 2800 Kongens Lyngby, Denmark;5. Department of Biology, Lund University, S?lvegatan 37, 223 62 Lund, Sweden;6. Neuronano Research Center, Lund University, S?lvegatan 19, 221 84 Lund, Sweden |
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Abstract: | Nanowires are commonly used as tools for interfacing living cells, acting as biomolecule‐delivery vectors or electrodes. It is generally assumed that the small size of the nanowires ensures a minimal cellular perturbation, yet the effects of nanowires on cell migration and proliferation remain largely unknown. Fibroblast behaviour on vertical nanowire arrays is investigated, and it is shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration. Using focused ion beam milling and scanning electron microscopy, highly curved but intact nuclear membranes are observed, showing no direct contact between the nanowires and the DNA. The nanowires possibly induce cellular stress and high respiration rates, which trigger the formation of ROS, which in turn results in DNA damage. These results are important guidelines to the design and interpretation of experiments involving nanowire‐based transfection and electrical characterization of living cells. |
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Keywords: | cell motility cell proliferation nanotoxicity nanowires reactive oxygen species |
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