结核菌H37Ra在小鼠体内诱导的免疫应答 |
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引用本文: | 刘来成,王淑玲,范雄林,付瑞玲,刘岚,卢贤瑜. 结核菌H37Ra在小鼠体内诱导的免疫应答[J]. 中国生物制品学杂志, 2008, 21(5): 391-394 |
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作者姓名: | 刘来成 王淑玲 范雄林 付瑞玲 刘岚 卢贤瑜 |
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作者单位: | [1]重庆医科大学儿童医院检验科,重庆400014 [2]重庆医科大学医学检验系,重庆400016 [3]华中科技大学同济医学院微生物学教研室,武汉430030 |
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摘 要: | 目的检测结核菌H37Ra免疫小鼠后产生的特异性细胞免疫和体液免疫应答水平。方法将BALB/c小鼠随机分为H37Ra组、BCG组和生理盐水(NS)组,分别进行免疫,免疫8周后处死小鼠,分离血清,ELISA间接法测定血清特异性抗PPDIgG抗体的水平,流式细胞分析仪检测脾脏T淋巴细胞亚群的变化。脾淋巴细胞经体外培养、PPD刺激后,MTT法检测脾淋巴细胞的刺激指数,ELISA法检测培养上清液中IFN-γ和IL-4的水平。结果H37Ra免疫小鼠血清中抗PPDIgG抗体、脾脏CD3+T细胞和CD4+T细胞的百分率、脾淋巴细胞刺激指数、IFN-γ和IL-4水平均显著高于NS对照组,但与BCG组差异无显著意义。各组间脾脏CD8+T细胞、CD4+T/CD8+T比值差异均无显著意义。结论H37Ra免疫小鼠后,可以产生特异性的细胞免疫和体液免疫应答,有望成为结核疫苗的候选抗原。
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关 键 词: | 结核分枝杆菌 H37Ra 细胞免疫应答 体液免疫应答 |
文章编号: | 1004-5503(2008)05-0391-04 |
修稿时间: | 2007-11-08 |
Immune Response Induced by Mycobacterium tuberculosis H37Ra in Mice |
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Abstract: | Objective To determine the specific cellular and humoral immune response levels induced by Mycobacterium tuberculosis H37Ra in mice.Methods Divide BALB/c mice into three groups and immunize by H37Ra,BCG and normal saline (NS) respectively.Kill the mice 8 weeks after immunization and determine the specific IgG level against PPD in sera by ELISA,and the T lymphocyte subgroup in spleens by flow cytometry.Culture splenic lymphocytes in vitro,stimulate with PPD and determine the stimulating index by MTT method,and the IFN-γ and IL-4 levels in culture supernatant by ELISA.Results The specific IgG level against PPD in sera,the percentages of CD3+ and CD4+ T lymphocytes in spleen,the stimulating index of splenic lymphocytes as well as IFN-γ and IL-4 levels of mice immunized by H37Ra was significantly higher than those by NS,while showed no significant difference with those by BCG.However,no significant difference was observed in the percentage of CD8+T lymphocytes or the ratio of CD4+T lymphocytes to CD8+T lymphocytes of various groups.Conclusion H37Ra induced specific cellular and humoral immune responses in mice and might be used as a candidate antigen for development of tuberculosis vaccine. |
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Keywords: | Mycobacterium tuberculosis H37Ra Cellular immune response Humoral immune response |
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