Complete separation of phospholipids from human heart combining two HPLC methods |
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| Authors: | Juanéda Pierre Rocquelin Gérard |
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| Affiliation: | (1) Station de Recherches sur la Qualité des Aliments de l'Homme, Institut National de la Recherche Agronomique, BV 1540, 21034 Dijon Cedex, France |
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| Abstract: | The separation of phospholipid classes from human heart was achieved in two steps by high performance liquid chromatography
(HPLC) using a silica column with an ultraviolet spectromonitor at 206 nm. A complete partitioning of phosphatidylcholines
(PC), phosphatidylethanolamines (PE), phosphatidylinositols (PI), phosphatidylserines (PS), cardiolipins (CL), lysophosphatidylcholines
(LPC) and sphingomyelins (Sph) was obtained for further analysis. |
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