| 1dhA基因敲除对Corynebacterium glutamicum TCCC11822 发酵生产L-谷氨酸的影响 |
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| 引用本文： | 张成林,李志华,梁静波 等. 1dhA基因敲除对Corynebacterium glutamicum TCCC11822 发酵生产L-谷氨酸的影响[J]. 中国酿造, 2014, 0(4): 106-109 |
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| 作者姓名： | 张成林 李志华 梁静波 等 |
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| 作者单位： | [1]代谢控制发酵技术国家地方联合工程实验室,天津300457 [2]天津市氨基酸高效绿色制造工程实验室,天津300457 [3]天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 国家高技术研究发展计戈旷863计划”（No.2013AA102106） |
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| 摘 要： | 针对L-谷氨酸发酵过程中乳酸产量偏高的问题,以L-谷氨酸生产菌谷氨酸棒杆菌（Corynebactenum glutamicum） TCCC 11822为出发菌株,通过构建敲除质粒pK18mobsacB△1dh并采用同源重组技术敲除其乳酸脱氢酶编码基因1dhA,以期达到减少副产物、提高L-谷氨酸产量和转化率的目的.结果表明,与出发菌株相比1dhA基因敲除株的乳酸合成量降低了85.6％,L-谷氨酸的产量和转化率分别提高7.6％和5.5％,但生物量略有下降.本研究可为L-谷氨酸及其他氨基酸生产菌株的理性改造提供参考.
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| 关 键 词： | L-谷氨酸 乳酸 乳酸脱氢酶 基因敲除 |
Impact of 1dhA knockout on L-glutamate fermentation by Corynebacterium glutamicum TCCC 11822 |
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| ZHANG Chenglin,LI ZhiHua,LIANG Jingbo,XU Qingyang. Impact of 1dhA knockout on L-glutamate fermentation by Corynebacterium glutamicum TCCC 11822[J]. China Brewing, 2014, 0(4): 106-109 |
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| Authors: | ZHANG Chenglin LI ZhiHua LIANG Jingbo XU Qingyang |
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| Affiliation: | 1.National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin 300457, China; 2. Tianjin Engineering Laboratory of Efficient and Green Amino Acid Manufacture, Tianjin 300457, China; 3.College of Biotechnology, Tianjin Universi(y of Science and Technology, Tianjin 300457, China) |
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| Abstract: | Lactic acid is one of main by products excreted in to the fermentation medium by Corynebacterium glutamicurn. To improve L-glutamate production and reduce L-lactate accumulation, pK18mobsacBAldh used for dehydrogenase-encoding gene ldhA knockout was constructed and ldhA was knocked out from L-glutamate producing strain Corynebacterium glutamicum TCCC11822, designated TCCC11822△1dh. Result showed that the lactate synthetic quantity reduced 85.6%, but the production and conversion of L-glutamate increased 7.6% and 5.5%, respectively. The biomass declined slightly. This study will lay theoretical foundation on rational reconstruction of L-glutamate and other amino acids producing strains. |
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| Keywords: | L-glutamate lactic acid lactate dehydrogenase gene knockout |
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