| 重组大肠杆菌流加发酵中短暂溶氧休克促进5-氨基乙酰丙酸生成(英文) |
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| 引用本文： | 杨俊,朱力,傅维琦,林逸君,林建平,岑沛霖. 重组大肠杆菌流加发酵中短暂溶氧休克促进5-氨基乙酰丙酸生成(英文)[J]. 中国化学工程学报, 2013, 21(11): 1291-1295. DOI: 10.1016/S1004-9541(13)60627-8 |
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| 作者姓名： | 杨俊 朱力 傅维琦 林逸君 林建平 岑沛霖 |
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| 作者单位： | 1.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;2.Center for Systems Biology, University of Iceland, Reykjavik, Iceland |
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| 基金项目： | Supported by the National Natural Science Foundation of China(20306026 and 20876141);the National Basic Research Program of China(2007CB707805) |
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| 摘 要： | 5-Aminolevulinic acid （ALA） is a common precursor for tetrapyrrole compounds in all kinds of organ isms and has wide applications in agriculture and medicines. In this study, a new strategy, i.e. short-term dissolved oxygen （DO） shock during aerobic fermentation, was introduced to produce 5-aminolevulinic acid with a recombi-nant E. coli. Effects of duration time of DO shock operation on plasmid concentration, intracellular ALA synthase （ALAS） activity and ALA production were investigated in Erlenmeyer shake flasks. The results indicated that both ALAS activity and ALA yield were enhanced in an anaerobic operation of 45 rain in the early exponential phase during fermentation, while they decreased when the anaerobic operation time was further increased to 60 rain. The DO shock protocol was confirmed with the fed-batch fermentation in a 15 L fermenter and the ALA production achieved 9.4 g.L-1 （72 mmol.L-1）, which is the highest yield in the fermentation broth reported up to now.
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| 关 键 词： | 5-aminolevulinic acid synthase activity dissolved oxygen shock fed-batch fermentation recombinant E. coli |
| 收稿时间： | 2012-06-04 |
Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation |
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| YANG Jun;ZHU Li;FU Weiqi;LIN Yijun;LIN Jianping;CEN Peilin. Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation[J]. Chinese Journal of Chemical Engineering, 2013, 21(11): 1291-1295. DOI: 10.1016/S1004-9541(13)60627-8 |
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| Authors: | YANG Jun ZHU Li FU Weiqi LIN Yijun LIN Jianping CEN Peilin |
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| Affiliation: | 1.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;2.Center for Systems Biology, University of Iceland, Reykjavik, Iceland |
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| Abstract: | 5-Aminolevulinic acid (ALA) is a common precursor for tetrapyrrole compounds in all kinds of organisms and has wide applications in agriculture and medicines. In this study, a new strategy, i.e. short-term dissolved oxygen (DO) shock during aerobic fermentation, was introduced to produce 5-aminolevulinic acid with a recombinant E. coli. Effects of duration time of DO shock operation on plasmid concentration, intracellular ALA synthase (ALAS) activity and ALA production were investigated in Erlenmeyer shake flasks. The results indicated that both ALAS activity and ALA yield were enhanced in an anaerobic operation of 45 min in the early exponential phase during fermentation, while they decreased when the anaerobic operation time was further increased to 60 min. The DO shock protocol was confirmed with the fed-batch fermentation in a 15 L fermenter and the ALA production achieved 9.4 g·L-1 (72 mmol·L-1), which is the highest yield in the fermentation broth reported up to now. |
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| Keywords: | 5-aminolevulinic acid synthase activity dissolved oxygen shock fed-batch fermentation recombinantE. coli |
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