Adsorption characteristics of thiobacillus ferrooxidans on surface of sulfide minerals |
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Authors: | Liu Jian-she Xie Xue-hui Li Bang-mei and Dong Qing-hai |
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Affiliation: | (1) School of Resources Processing and Bioengineering, Central South University, 410083 Changsha, China;(2) General Research Institute for Nonferrous Metals, 100088 Beijing, China |
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Abstract: | By using thiobacillus ferroxidans (T. f) from Qixiashan, Hubei Province, China, the adsorption characteristics of T. f on surface of sulfide mineral were studied. The influences of adsorption time, pH value, temperature, initial inoculated
concentration of bacteria, concentration of sulfide mineral powder, and variety of minerals on the adsorption characteristics
were firstly investigated by using the ninhydrin colorimetric method, and the changes of contact angles and Zeta potentials
of mineral surface during the bacterial adsorption were then determined. The results show that when the leaching experiments
are performed for a long time from several days to a month, the maximal quantity of adsorption of T f on the surface of pyrite is obtained under the following conditions: leaching for 20 d, pH value in range of 1–2 and temperature
at 30 °C, respectively; when the bio-leaching experiments are performed for a shorter leaching time, the maximal quantity
of adsorption is obtained under the conditions: bio-leaching for 2 h, at 2.4×107 cell/mL of initial inoculated bacteria concentration, and at 10% of mineral powder concentration; and the adsorption quantities
are different from one sulfide mineral to another, and the adsorption of T. f on the surface of sulfide minerals includes three phases: increasing phase, stationary phase and decreasing phase.
Foundation item: Project(50321402) supported by the National Natural Science Foundation of China; project (2004CB619204) supported by the
National Key Fundamental Research and Development Program of China |
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Keywords: | thiobacillus ferrooxidans sulphide mineral adsorption bio-leaching |
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