| 一株高产乳酸细菌的分离鉴定与发酵性能研究 |
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| 引用本文： | 王旭明,汪群慧,任南琪,王孝强.一株高产乳酸细菌的分离鉴定与发酵性能研究[J].哈尔滨工业大学学报,2006,38(9):1483-1486,1495. |
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| 作者姓名： | 王旭明 汪群慧 任南琪 王孝强 |
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| 作者单位： | 哈尔滨工业大学,市政环境工程学院,哈尔滨,150090;吉林大学,植物科学学院,长春,130062;哈尔滨工业大学,市政环境工程学院,哈尔滨,150090;北京科技大学,环境工程系,北京,100083;哈尔滨工业大学,市政环境工程学院,哈尔滨,150090 |
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| 基金项目： | 国家自然科学基金资助项目(50278024)，黑龙江省自然科学基金资助项目(E0316) |
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| 摘 要： | 从食物垃圾中分离到1株乳酸高产菌株TD175,该菌株在含100 g/L葡萄糖的发酵培养基中,经72 h发酵,可产生78.56 g/L的乳酸.根据形态和生理生化特征,将菌株TD175初步鉴定为乳杆菌属(Lactobacillussp.)的细菌,其16S rDNA序列与乳杆菌MR2菌株、植物乳杆菌(L.plan tarum)和戊糖乳杆菌(L.pen tosus)的相似性最高,均达到99%.菌株TD175经过耐酸选育得到的新菌株TD175-1的乳酸产量提高了10.7%.菌株TD175-1能促进食物垃圾的乳酸发酵,厌氧发酵48 h,产生29.65 g/L的乳酸,比不接种的自然发酵高34.3%.
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| 关 键 词： | 乳酸 乳酸细菌 发酵 鉴定 |
| 文章编号： | 0367-6234（2006）09-1483-04 |
| 收稿时间： | 2005-04-21 |
| 修稿时间： | 2005-04-21 |
Isolation and identification of a high-yield lactic acid bacterium and its fermentative performance |
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| WANG Xu-ming,WANG Qun-hui,REN Nan-qi,WANG Xiao-qiang.Isolation and identification of a high-yield lactic acid bacterium and its fermentative performance[J].Journal of Harbin Institute of Technology,2006,38(9):1483-1486,1495. |
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| Authors: | WANG Xu-ming WANG Qun-hui REN Nan-qi WANG Xiao-qiang |
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| Affiliation: | 1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; 2. School of Plant Science, Jilin University, Changchun 130062, China ; 3. Dept. of Environmental Engineering, Beijing University of Science and Technology, Beijing 100083, China |
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| Abstract: | A strain of high-yield lactic acid bacterium,designated as TD175,was isolated from food waste.The strain TD175 produced 78.56 g/L of lactic acid from 100 g/L of glucose after 72 h fermentation.Based on its morphology and physio-biochemical properties,the strain TD175 was tentatively identified as Lactobacillus sp..Analysis of 16S rDNA sequence showed that TD175 had high homology(99%) with Lactobacillus sp.MR2,L.plantarum and L.pentosus.An acid-tolerance strain TD175-1 was obtained by breeding of the strain TD175.Lactic acid output for the strain TD175-1 was 10.7% higher than the strain TD175.The strain TD175-1 was capable of enhancing lactic acid fermentation of food waste,with 29.65 g/L of lactic acid produced after 48 h of anaerobic fermentation,which was 34.3% higher than the spontaneous fermentation without inoculum. |
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| Keywords: | lactic acid lactic acid bacterium fermentation identification |
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