Single-lens theta microscopy — a new implementation of confocal theta microscopy |
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| Authors: | S. Lindek,T. Stefany,& E. H. K. Stelzer |
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| Affiliation: | Light Microscopy Group, Cell Biophysics Programme, European Molecular Biology Laboratory (EMBL), Postfach 102209, D–69012 Heidelberg, Germany |
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| Abstract: | A new microscopical technique based on the principle of confocal theta microscopy (Stelzer, E.H.K., Lindek, S. & Pick, R. (1996) Konfokales Mikroskop . German Patent Office DE 43 26 473 (filed 6.8.1993, granted 6.12.1996)) is described. It uses a single objective lens in combination with a mirror unit to achieve the theta configuration that leads to axial and volume resolution improvements. In this paper we present technical details of possible microscopical set-ups, and we discuss different versions of mirror units. |
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| Keywords: | 4Pi microscopy confocal fluorescence numerical aperture resolution theta microscopy water-immersion objective lenses |
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