葡萄糖和蛋白胨对绿色木霉纤维素酶系分泌特性的影响

采用正交试验法分析出绿色木霉3.3711液体发酵产纤维素酶系各组分的最适酶解条件,其组合分别为C1(pH 5.0、45℃),Cx(pH 3.5、55℃)和βG(pH 4.5、65℃)。在液体基础产酶培养基发酵条件下,三种组分酶的最高酶活分别为53.6、185.2、79.3 u/mL,比酶活分别为46.6、167.7、71.8 u/mg,最高的酶活形成时间分别为9、10、10 d。添加质量分数0.0005~0.003的葡萄糖,三种组分酶最高酶活分别降低了27.4%~38.4%、25.5%~35.5%、14.0%~52.3%,C1和Cx的最高酶活形成时间分别缩短为4~7、5 d,C1和βG比酶活分别降低了20.8%~35.4%、25.8%~64.5%;添加质量分数0.0005~0.01的蛋白胨,提高了C1和Cx最高酶活(分别为0.9%~199.6%、2.1%~45.1%),缩短了C1和Cx的最高酶活形成时间(分别为4~7、4 d),降低了βG的最高酶活(5.9%~34.2%)和比酶活(35.2%~87.3%),对C1和Cx比酶活的增减效应存在浓度依赖性,质量分数0.0005时分别提高50.0%、65.4%。两种添加物均能明显改变C1和Cx的分泌变化规律,对βG酶影响极小。

Effects of Glucose and Peptone on Characteristics of Cellulase System Induced by Trichoderma Viride

By adopting orthogonal test, the optimal enzymolysis condition for respective components of cellulase system from Trichoderma viride 3. 3711 induced liquid fermentation are found to be pH 5.0, 45 ℃ for enzyme C1, pH 3.5, 65 ℃ for enzyme Cx, and pH 4.5, 65 ℃ for enzyme βG. Under the condition of liquid state and basic cultivation fermentation, the highest enzyme activity (HEA) of the three components are 53.6, 185.2 and 79.3 u/mL, enzyme activity ratio (EAR) are 46.6, 167.7 and 71.8 u/mg, formation time of highest enzyme activity (FTHEA) are 9, 10 and 10 d, respectively. Addition of 0.05%～0.3% glucose leads to decreasing HEA of the three components by 27.4%～38.4%, 25.5%～35.5% and 14.0%～52.3%, shortening FTHEA of C1 and Cx to 4～7 and 5 d, and decreasing EAR of C1 and βG by 20.8%～35.4% and 25.8%～64.5%, respectively. Addition of 0.05%～1.0% peptone leads to increasing HEA of CI and Cx by 0.9%～199.6% and 2.1%～45.1%, shortening FTHEA of C1 and Cx to 4～7 and 4 d, and decreasing HEA and EAR of βG by 5.9%～34.2% and 35.2%～87.3%, respectively. The effects of peptone on EAR of C1 and Cx are concentration dependent and concentration 0.05% leads to increasing the effects by 50.0% and 65.4%, respectively. Regarding to the rule of enzyme secretion of respective components, glucose and peptone both show a certain effect on C1 and Cx and few effect on βG.