桃色顶孢霉发酵液对大豆的促生及对抗氧化酶活性的影响

采用管碟法和种子萌发法研究桃色顶孢霉对茄病镰刀菌Fusarium solanae的拮抗作用以及对大豆的促生作用，并采用愈创木酚法、紫外吸收法、氮蓝四唑光化还原法研究了顶孢霉发酵液对POD、CAT、SOD三种抗氧化酶活性的影响。结果表明：顶孢霉发酵液对镰刀菌菌丝生长有明显抑制作用，菌丝变稀疏。大豆种子经不同浓度桃色顶孢霉发酵液处理后，萌发率及种子酶活性均产生变化。不同浓度的发酵液对种子萌发促进作用不同，当发酵液浓度为10％时，胚根长、胚芽长、胚根鲜重和胚芽鲜重均达到最高值，分别为2．91cm、0．037g、4．07cm 和0．1666g，与对照相比分别提高了49．37％、63．24％、30．54％和45．80％。当处理种子的发酵液浓度为60％时，种子萌发率最高为83．67％，与对照相比提高了23．90％；POD、CAT和 SOD 酶活性最强，分别为683U、160．6U 和301U，与对照相比分别提高73．50％、43．78％和76．74％。初步证实了桃色顶孢霉发酵液中含有能够促进大豆种子萌发及抗氧化酶活性升高的物质。

Effect of Acremonium persicium fermentation on seeds germination and antioxidan enzymes activity of soybean

In this study,the antagonism ability of fermentation of Acremonium persicinum to Fusarium solanae and the growth promoting ability on soybean germination process were studied by cylinder-plate method and seed germination method.Guaiacol,UV spectrophotometric ,NBT-law were used to determine POD,CAT,SOD of soybean seed to evaluate effects of fermentation of A.persicium.The result showed that the hypha growth of F.sola-nae was inhibited by the fermentation of A.persicinum,and the hypha became less dense.The germination rate and enzyme activity of soybean seeds treated by different concentration of fermentation were all changed.The affectivity were different,when treated by the fermentation at the concentration of 10%,the length of radicle and hypocotyl, the raw weight of radicle and hypocotyl were all at the highest value,increased by 49.37%,63.24%,30.54%and 45 .80% respectively comparing to control.When treated by the fermentation at the concentration of 60%,the germination rate was the highest,up to 83.67%;the POD,CAT and SOD enzyme activities were 683U,160.67U and 301U respectively,increased by 73.50%,43.78%and 76.74%comparing to control.It was confirmed that there were some constituents could promote soybean seeds germination and increase anti-stress enzyme activities in the A.persicinum fermentation.