烟草中挥发性与半挥发性有机酸的GC-MS法快速检测

建立了气相色谱-质谱联用同时测定烟草中25种挥发性与半挥发性有机酸的快速定量分析方法。样品经二氯甲烷震荡萃取,N,O-双(三甲基硅基)三氟乙酰胺(BSTFA)硅烷化,以反-2-己烯酸和肉桂酸双内标法定量,利用GC-MS/SIM法同时测定了25种挥发性、半挥发性有机酸。结果表明:此方法测定有机酸具有良好的线性(r2≥0.985)和重复性(RSDs≤8.93%,n=5),平均回收率81.43%~100.58%,检测限为0.03~2.30μg/g,定量限为0.10~7.67μg/g。建立的方法简便快捷,且具有较高的准确度和精密度,适用于烟草中挥发性与半挥发性有机酸的快速和同时测定。

Rapid Determination of Volatile and Semi-Volatile Organic Acids in Tobacco by GC-MS

A rapid quantitative analysis method for simultaneously determining 25 volatile and semi-volatile organic acids in tobacco by GC-MS was established on the basis of optimized parameters including reagent volume,time and temperature in derivatization,etc.The organic acids in tobacco were extracted with dichloromethane and derived with N,O-bis（trimethylsilyl）trifluoroacetamide.The derivatives of 25 organic acids,including formic acid,acetic acid and propanoic acid etc.,were simultaneously determined by GC-MS with selective ion mode,using trans-2-hexenoic acid and cinnamic acid as dual internal standards.Good linearity （r2≥0.985） and good repeatability （RSDs≤8.93％,n =5） for 25 organic acids in sample were achieved.The recoveries of the method ranged from 81.43％ to 100.58％.The limits of detection ranged from 0.03 μg/g to 2.30 μg/g and the limits of quantification ranged from 0.10μg/g to 7.67 μg/g.This method was suitable for rapid and simultaneous analysis of volatile and semi-volatile organic acids in tobacco,and it was simple,rapid,effective and accurate.