| 正交试验优化超声辅助法提取三七有效成分工艺 |
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| 引用本文： | 周国燕,王春霞,桑迎迎,胡晓亮.正交试验优化超声辅助法提取三七有效成分工艺[J].食品科学,2012,33(12):8-11. |
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| 作者姓名： | 周国燕 王春霞 桑迎迎 胡晓亮 |
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| 作者单位： | 上海理工大学低温生物与食品冷冻研究所 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(50206013);上海市教委科研创新项目(09YZ230);上海市重点学科建设项目(S30503);上海理工大学医疗器械与食品学院微创励志创新基金项目 |
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| 摘 要： | 研究超声辅助法提取三七有效成分的工艺。通过单因素试验研究乙醇溶液体积分数、料液比、超声波频率和提取时间对三七人参皂苷Rg1提取率的影响。在单因素试验基础上对4个因素安排L9(34)正交试验，得出影响三七有效成分人参皂苷Rg1提取率的先后次序为：乙醇体积分数＞提取时间＞料液比＞超声频率。选出最佳提取工艺为：乙醇体积分数70%、料液比1:12(g/mL)、超声频率59kHz、提取时间60min。在此最佳工艺下，得到三七干燥后的人参皂苷Rg1含量为1.85%。
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| 关 键 词： | 三七 超声辅助法 人参皂苷Rg1 提取 |
Orthogonal Array Optimization of Ultrasonic-Assisted Extraction of Panax notoginseng Saponin Rg1 |
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| ZHOU Guo-yan,WANG Chun-xia,SANG Ying-ying,HU Xiao-liang.Orthogonal Array Optimization of Ultrasonic-Assisted Extraction of Panax notoginseng Saponin Rg1[J].Food Science,2012,33(12):8-11. |
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| Authors: | ZHOU Guo-yan WANG Chun-xia SANG Ying-ying HU Xiao-liang |
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| Affiliation: | (Institute of Cryo-medicine and Food Refrigeration, Shanghai University of Science and Technogy, Shanghai 200093, China) |
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| Abstract: | The ultrasonic-assisted extraction of Panax notoginseng saponin Rg1 was optimized using an L9(34) orthogonal array design based on one-factor-at-a-time experiments.The effects of ethanol concentration,material-to-liquid ratio,ultrasonic frequency and extraction time on extraction efficiency were examined.Ethanol concentration had the highest effect on extraction efficiency,followed by extraction time and material-to-liquid ratio;the effect of ultrasonic frequency was the weakest.The optimal levels of ethanol concentraion,material-to-liquid ratio(g/mL),ultrasonic frequency and extraction time were 70%,1:12,59 kHz and 60 min,respectively.Under the optimized extraction conditions,the extraction yield of saponin from Radix Panax notoginseng was 1.85%. |
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| Keywords: | Panax notoginseng ultrasonic-assisted extraction saponin Rg1 |
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