| 光谱法研究绿原酸和牛血清白蛋白的相互作用 |
| |
| 引用本文： | 孙艳辉,魏亚玲,吴霖生,张汆.光谱法研究绿原酸和牛血清白蛋白的相互作用[J].食品工业科技,2010(4). |
| |
| 作者姓名： | 孙艳辉 魏亚玲 吴霖生 张汆 |
| |
| 作者单位： | 滁州学院化学与生命科学系,安徽,滁州,239012 |
| |
| 基金项目： | 安徽省应用化学重点学科基金项目资助; 安徽高等学校省级自然科学研究项目(KJ2008B227); 滁州市重点科技计划项目(2008036) |
| |
| 摘 要： | 为了解绿原酸和牛血清白蛋白的相互作用,在模拟动物生理条件下,采用荧光光谱和紫外-可见吸收光谱结合Stern-Volmer方程、double-reciprocal方程和热力学公式来研究绿原酸与牛血清白蛋白相互作用特征。结果表明:绿原酸和牛血清白蛋白在298K和310K两个温度下的结合常数分别为1.869×105L·mol-1和1.574×105L·mol-1;结合位点分别为1.18和1.16;且热力学参数ΔH0、ΔS0、ΔG0;结合距离小于7nm。因此,绿原酸对牛血清白蛋白有较强的荧光静态猝灭作用,符合静态猝灭过程,结合力为疏水作用。
|
| 关 键 词： | 绿原酸 牛血清白蛋白 相互作用 荧光光谱 紫外光谱 |
Study on the interaction of chlorogenic acid and bovine serum albumin by spectrum |
| |
| SUN Yan-hui,WEI Ya-ling,WU Ling-sheng,ZHANG Cuan.Study on the interaction of chlorogenic acid and bovine serum albumin by spectrum[J].Science and Technology of Food Industry,2010(4). |
| |
| Authors: | SUN Yan-hui WEI Ya-ling WU Ling-sheng ZHANG Cuan |
| |
| Affiliation: | SUN Yan-hui,WEI Ya-ling,WU Ling-sheng,ZHANG Cuan (Department of Chemistry , Life Sciences,Chuzhou University,Chuzhou 239012,China) |
| |
| Abstract: | To study the interaction of chlorogenic acid and bovine serum albumin,the fluorescence spectroscopy, UV-visible absorption spectroscopy, Stern- Volmer equation, double- reciprocal equation and formula of thermodynamics were used to obtain binding characteristics of the interaction in a simulated physiological conditions of animals.The results showed:the binding constants of chlorogenic acid and bovine serum albumin at 298.02K and 310.02K were 1.869×10~5 and 1.574×10~5L·mol~(-1),binding site were 1.18 and 1.16,respectively,and the thermodynamic parameters were △H >0, △S >0, △G <0.And their combined distance was less than 7nm. Chlorogenic acid on bovine serum albumin had a stronger role in fluorescence quenching, in line with the static quenching process for hydrophobic binding. |
| |
| Keywords: | chlorogenic acid cattle serum albumin interaction fluorescence spectrum UV-visible absorption spectrum |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
