Inverse protein folding by the residue pair preference profile method: estimating the correctness of alignments of structurally compatible sequences |
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Authors: | Wilmanns Matthias; Eisenberg David |
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Affiliation: | UCLA-DOE Laboratory of Structural Biology and Molecular Medicine and Molecular Biology Institute, University of California at Los Angeles Los Angeles, CA 90024-1570, USA
1Present address: European Molecular Biology Laboratory Meyerhofstrasse 1, Postfach 10.2209, D-69012 Heidelberg, Germany |
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Abstract: | The residue pair preference profile (R3P) method is an inversefolding method that combines environmental profiles and pairpreference profiles. The method uses statistical preferencesfor residue pairs which score the likelihood of finding a profiledresidue to be paired with a residue within its local environmentAll pairs are characterized by their dihedral angles, secondarystructure and number of neighboring residues as a function ofresidue type. Each residue pair preference is expressed forall 20 amino acids of the profiled residue and is weighted bythe compatibility of the environment residue with its own localenvironment The R3P method produces an initial profile-sequencealignment which is then refined by converting the initial profileinto a profile of a target sequence threaded into the structureof the initial profile. We have tested this method by evaluatingalignments of sequences with known 3-D structures using structuralsuperposition alignments as reference. R3P-sequence alignmentsare 50% correct on average for sequences whose 3-D structurepairs superimpose with an r.m.s. deviation of 1.97 Å.The average improvement in correctness during this iterativerefinement is 14%. The R3P-sequence alignments are comparedwith sequence-sequence and 3-D profile-sequence alignments.When all three methods are combined, on average 50% of the alignmentsare correct for pairs of 3-D structures that superimpose within2.12 Å. A 3-D model of HisA is predicted with the combinedmethod. |
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Keywords: | alignment/ HisA/ inverse protein folding/ pair potential/ profile |
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